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KMID : 0984720030350010012
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Infection and Chemotherapy
2003 Volume.35 No. 1 p.12 ~ p.17
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Use of RNA/RNA Duplex, Base Pair-mismatch Assay and 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Assay for Rapid Detection of Rifampin-resistant Mycobacterium tuberculosis
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Hong Sung-Kwan
Jang Gyeong-Hui
Park Yoon-Soo
Cho Cheong-Ho
Kim Hyo-Yeol
Song Young-Goo
Kim June-Myung
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Abstract
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Background: Multidrug-resistant tuberculosis is an increasing concern for public health in many parts of the world. We have evaluated the specificity and sensitivity of the mismatch assay and 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT) assay in detecting rifampin- resistant Mycobacterium tuberculosis.
Methods: Eleven rifampin-susceptible and 15 rifampin-resistant M. tuberculosis strains were isolated from clinical specimens obtained from patients in Yonsei University College of Medicine, Severance Hospital. RNA/RNA duplex, base pair-mismatch assay (Mismatch Detect II kit, Ambion) and MTT assay were performed.
Results: The specificity and sensitivity of detection of rifampin resistance were 91% and 87% in mismatch assay and 73% and 67% in MTT assay, respectively.
Conclusion:These results suggest the usefulness of mismatch assay in detecting rifampin-resistant Mycobacterium tuberculosis.
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KEYWORD
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rifampin-resistant mycobacterium tuberculosis, mismatch assay, MTT assay
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